Molecular characterization and allergenicity potential of triosephosphate
isomerase fromSarcoptes scabiei
Ran He, Haojie Zhang, Nengxing Shen, Cheng Guo,Yongjun Ren, Yue Xie, Xiaobin Gu,
Weimin Lai, Xuerong Peng, Guangyou Yang
Abstract
Scabies is an allergic skin disease that affects millions of mammals worldwide, including humans. It is a neglected tropical disease that represents a significant public health threat, particularly in economically disadvantaged populations. An effective vaccine is not currently available, and the exact mode of pathogenesis remains unclear. Herein, we identified, cloned and recombinantly expressed triosephosphate isomerase fromSarcoptes scabiei(S. scabiei). Immunohistochemical analyses showed thatS. scabieitriosephosphate isomerase (Ss-TIM) is localized in the legs and chewing mouthparts of mites, and in infected rabbit skin (keratinized skin and embedded mites). Intradermal skin tests of rabbits injected with recombinantS. scabieitriosephosphate isomerase (rSs-TIM) revealed a flare, erythema and wheal reaction. These findings suggest that Ss-TIM may contribute to host invasion and induce an allergic response in the host.
copyright:©2018 Published byElsevier B.V.
Veterinary Parasitology (2018). doi.org/10.1016/j.vetpar.2018.05.016
Read Full Text: https://doi.org/10.1016/j.vetpar.2018.05.016
