Cloning and characterization of the fatty acid-binding protein gene from the protoscolex ofTaenia multiceps
Nie HM, Xie Y, Fu Y, Yang YD, Gu XB, Wang SX, Peng X, Lai WM, Peng XR, Yang GY.
Abstract
Taeniamulticeps(Cestoda: Taeniidae), a worldwide cestode parasite, is emerging as an important helminthic zoonosis due to serious or fatal central nervous system disease commonly known as coenurosis in domestic and wild ruminants including humans. Herein, afattyacid-bindingprotein(FABP)genewas identified from transcriptomic data inT.multiceps. Thisgene, which contains a complete coding sequence, was amplified by reverse-transcriptase polymerase chain reaction. The correspondingprotein, which was named TmFABP, had a molecular weight of 14 kDa, and subsequently was recombinantly expressed inEscherichia coli. The fusionproteinwas purified on Ni-NTA beads (Bio-Rad). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analyses showed that the purified recombinantproteincaused immunogenicity. Immunohistochemical studies showed that TmFABP was expressed at the tegumental level in the protoscolices and in the cells between the body wall and parenchyma layer of the cestode. In sections from gravid proglottids, intense staining was detected in the uterus and eggs. Based on this, TmFABP could be switched on during differentiation of germinative layers to protoscoleces and from metacestodes to adult worms. Taken together, our results already reported forT.multicepssuggest the possibility of TmFABP developing a vaccine to control and prevent coenurosis.
Copyright ©Springer-Verlag Berlin Heidelberg 2013
Parasitol Res.2013 May;112(5):1833-9. doi: 10.1007/s00436-013-3328-0. Epub 2013 Mar 9.
Read full text:http://link.springer.com/article/10.1007%2Fs00436-013-3328-0
