Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells

点击数:1102022-11-04 13:01:00 

Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells

 Ziyi Xu, Yanting Xu, Xiaobin Gu, Yue Xie, Ran He, Jing Xu, Bo Jing, Xuerong Peng and Guangyou Yang

Abstract: Scabies is a common parasitic dermatological infection worldwide that is often neglected.Scabies mites stimulate host inflflammatory symptoms via secreted and excreted proteins, which induce basophil and mast cell degranulation and host histamine release. However, the mechanism of degranulation and histamine release is unclear. Moreover, the Sarcoptes scabiei translationally controlled tumor protein (TCTP) is predicted as an excreted protein, which may be involved in host inflflammatory response regulation. First, we evaluated S. scabiei TCTP gene (SsTCTP) transcription in larvae, nymphs, and adults by qRT-PCR, and SsTCTP transcription was highest in larvae, followed

by nymphs. Second, we found that the S. scabiei TCTP recombinant protein (rSsTCTP) promoted mice histamine release in vivo by Evans blue Miles assay. Therefore, to further explore the possible role of S. scabiei TCTP in host inflflammatory response regulation, we established a degranulation model of KU812 cells. The results of the degranulation model suggested that rSsTCTP could induce enhanced degranulation of KU812 cells and increase the secretion of histamine and the expression of IL-4, IL-6, and IL-13 in vitro. In conclusion, we speculate that scabies mites could stimulate host histamine release and Th2 response by excreting S. scabiei TCTP.

Keywords: histamine; KU812 cells; Sarcoptes scabiei; translationally controlled tumor protein
Citation: Xu, Z.; Xu, Y.; Gu, X.; Xie, Y.; He, R.; Xu, J.; Jing, B.; Peng, X.; Yang, G. Effects of Sarcoptes scabiei Translationally Controlled Tumor Protein (TCTP) on Histamine Release and Degranulation of KU812 Cells. Int. J. Mol. Sci. 202223, 12865. https://doi.org/10.3390/ijms232112865