Molecular Diagnosis ofBaylisascaris schroederiInfections in Giant Panda (Ailuropoda melanoleuca) Feces Using PCR
Zhou X, Yu H, Wang N, Xie Y, Liang YN, Li DS, Wang CD, Chen SJ, Yan YB, Gu XB, Wang SX, Peng XR, Yang GY.
Abstract
The helminthBaylisascarisschroederiis one of the most harmful parasites infectinggiantpandas (Ailuropodamelanoleuca). It is therefore important to develop an exact diagnostic technique to detect this parasite. Using a known number (1, 2, 3, 4, 5, 10, 25, 50, 100) offeces-isolated B.schroederiegg and adult DNA, we developed aPCRto detect a portion of the mitochondrial 12S rRNA and applied it togiantpandafecal samples. The method was sufficiently sensitive to detectB.schroederiDNA from isolated eggs in a fecal sample with a detection threshold of one egg. We detectedB.schroederiin 88% of fecal samples, 30% higher than the conventional flotation technique. No cross-reactivity with other common nematode DNA was detected. OurPCRassay may constitute a valuable alternative for thediagnosisofB.schroederiinfections.
Copyright ©Wildlife Disease Association 2013
J Wildl Dis.2013 Oct;49(4):1052-5. doi: 10.7589/2012-07-175.
Read full text: http://www.jwildlifedis.org/doi/pdf/10.7589/2012-07-175
