Annotation of the Transcriptome fromTaenia pisiformisand Its Comparative Analysis with Three Taeniidae Species
Yang D, Fu Y, Wu X, Xie Y, Nie H, Chen L, Nong X, Gu X, Wang S, Peng X, Yan N, Zhang R, Zheng W, Yang G.
Abstract
BACKGROUND:Taeniapisiformisis one of the most common intestinal tapeworms and can cause infections in canines. AdultT.pisiformis(canines as definitive hosts) and Cysticercuspisiformis(rabbits as intermediate hosts) cause significant health problems to the host and considerable socio-economic losses as a consequence. No complete genomic data regardingT.pisiformisare currently available in public databases. RNA-seq provides an effective approach to analyze the eukaryotictranscriptometo generate large functional gene datasets that can be used for further studies.
METHODOLOGY/PRINCIPAL FINDINGS:In this study, 2.67 million sequencing clean reads and 72,957 unigenes were generated using the RNA-seq technique. Based on a sequence similarity search with known proteins, a total of 26,012 unigenes (no redundancy) were identified after quality control procedures via the alignment of four databases. Overall, 15,920 unigenes were mapped to 203 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Through analyzing the glycolysis/gluconeogenesis and axonal guidance pathways, we achieved an in-depth understanding of the biochemistry ofT.pisiformis. Here, we selected four unigenes at random and obtained their full-length cDNA clones using RACE PCR. Functional distribution characteristics were gained through comparing four cestodespecies(72,957 unigenes ofT.pisiformis, 30,700 ESTs ofT. solium, 1,058 ESTs of Eg+Em [conserved ESTs between Echinococcus granulosus and Echinococcus multilocularis]), with the cluster of orthologous groups (COG) and gene ontology (GO) functional classification systems. Furthermore, the conserved common genes in these four cestodespecieswere obtained and aligned by the KEGG database.
CONCLUSION:This study provides an extensivetranscriptomedataset obtained from the deep sequencing ofT.pisiformisin a non-model whole genome. The identification of conserved genes may provide novel approaches for potential drug targets and vaccinations against cestode infections. Research can now accelerate into the functional genomics, immunity and gene expression profiles of cestodespecies
Copyright ©2012 Yang et al.
PLoS One.2012;7(4):e32283. doi: 10.1371/journal.pone.0032283. Epub 2012 Apr 13.
Read full text:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032283#pone-0032283-g005
