Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia multiceps
Yuchen Liu, Yingdong Yang, Jing Xu, Xiaowei Dong, Xiaobin Gu, Yue Xie, Weimin Lai, Bo Jing, Xuerong Peng, Guangyou Yang
Abstract
Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode ofTaenia multiceps(Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development ofappropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B(AgB) and thioredoxin peroxidase (TPx) fromT. multicepswere cloned and expressed using a prokaryotic system,molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnosticpotentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previouslyreported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein ofcestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% anda specificity of 87.5%, indicating its strong potential for serological diagnosis ofT. multiceps.
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Veterinary Parasitology 272 (2019) 58–63.doi.org/10.1016/j.vetpar.2019.07.002
Read Full Text: https://doi.org/10.1016/j.vetpar.2019.07.002
