Molecular characterisation and expression analysis of
two heat-shock proteins in Taenia multiceps
Yuchen Liu, Cheng Guo, Xiaowei Dong, Xiaobin Gu, Yue Xie, Weimin Lai, Xuerong Peng and Guangyou Yang
Abstract
Background: Taenia multicepsis a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular chaperones and can affect pathogenicity.
Methods: Herein, cDNAs ofT. multicepsgenes Tm-HSP60 and Tm-p36 were cloned and molecularly characterised by bioinformatics analyses. The immunogenicity and immunoreactivity of recombinant rTm-HSP60 and rTm-p36 proteins were investigated by immunoblotting and indirect ELISA was established to evaluate their serodiagnostic potential. Tissue localisation and transcriptional level at different life stages ofT. multicepswere determined by immunohistochemical and quantitative real-time PCR analyses.
Results: The 533 residue rTm-HSP60 and the 314 residue rTm-p36 proteins share typical highly conserved features of HSPs. Tm-p36 shares structural characteristics with metazoan small HSPs, with two N-terminal α-crystallin domains. Compared with Tm-p36, Tm-HSP60 displayed stronger immunogenicity, and the indirect ELISA based on rTm-HSP60 exhibited a sensitivity of 83.3% and a specificity of 87.5%, while rTm-p36 was not suitable to develop indirect ELISA. Tm-HSP60 was widely distributed in all stages ofT. multiceps, albeit at relatively low levels, while Tm-p36 was specifically distributed in the protoscolex and oncosphere.
Conclusion: The sequence, structural and functional analyses of these two HSPs indicates that they may play important roles in the life-cycle ofT. multicepsas molecular chaperones. Tm-HSP60 displayed stronger immunogenicity compare to Tm-p36, and has the potential for antibody detection. Tm-p36 was strongly associated with the activation of oncospheres and has potential interest for vaccination.
copyright:© The Author(s).
Parasites & Vectors (2019) 12:93. DOI: 0.1186/s13071-019-3352-8
Read Full Text: https://doi.org/10.1186/s13071-019-3352-8
