Molecular characteristics and serodiagnostic potential ofchitinase-likeprotein
fromSarcoptes scabiei
Ran He, Nengxing Shen, Haojie Zhang, Yongjun Ren, Manli He, Jing Xu,Cheng Guo, Yue Xie,Xiaobin Gu,
Weimin Lai,Xuerong Peng and GuangyouYang
Abstract
Scabies, caused by the miteSarcoptes scabiei, is an allergic skin disease thataffects millions of people and other mammals worldwide. This highly contagiousparasitic disease is among the top 50 epidemic disease and is regarded as aneglected tropical disease. Diagnosis of scabies is diffcult in the early stage, and thepathogenesis of scabies is not currently clear. Here, we expressed, identifed andlocated the chitinase-like protein ofS. scabiei(SsCLP), and evaluated its potential asan early-stage diagnostic antigen for rabbit scabies. Indirect ELISA using recombinantSsCLP (rSsCLP) exhibited diagnostic sensitivity of 94.4% (17/18) and specifcity of86.7% (26/30). Early diagnostic test after artifcial infection of rabbits withS. scabiei for 1 week showed a positive detection rate of 96.7% (29/30). Immunolocalizationassays showed that fluorescence signals were localized on the surface of mites and, ininfected rabbits, were observed in keratinized skin and embedded mites. Intradermalskin tests of rabbits by injecting rSsCLP showed a wheal, flare and erythema reaction.These results suggest thatS. scabieichitinase-like protein is conducive to hostinvasion, participates in inducing the allergic response of the host, and is an effectiveantigen for the diagnosis ofS. scabiei.
copyright:©He et al.
Oncotarget, (2017).DOI: 10.18632/oncotarget.21056
ReadFull Text: www.researchgate.net/publication/319913060
