Genetic variation in mitochondrial cox2 ofHeterakis gallinarum from poultryin
Sichuan,China
Xiao-Bin Gu, Bao-Jian Wang, Xi-Bin Zhao, Yan-Fang Li, Guang-You Yang, WeiMing Lai, Zhi-Jun Zhong
& Guang-Neng Peng
Abstract
Heterakis gallinarumis one of the common parasitic nematodes found in the caecum of poultry. Toinvestigate the genetic diversity and genetic structure of theH. gallinarumpopulation in Sichuan, weamplified and sequenced the complete mitochondrial (mt) cytochrome c oxidase subunit II (cox2) geneof 59H. gallinarumisolates from seven different geographical regions, then analyzed their genetic polymorphisms. Allcox2genes of the 59H. gallinarumisolates were 696 bp in length, with an averageA+T content of 67.1%. Fifty-nine sequences contained 34 variable sites, and were classified into 23haplotypes (HS1–HS23). The values of haplotype diversity (Hd) and nucleotide diversity (p) were0.688 and 0.00288, respectively. Based on values ofFSTandNm(FST=0.01929,Nm=12.71), there was afrequent gene flow but no significant genetic differentiation observed among the populations. The network map showed that the most prominent haplotype was HS1, and the other haplotypes (HS2–HS23)were centered on HS1 with a star-like topology, indicating thatH. gallinarumhad previously experienced a population expansion. To our knowledge, this is the first research on the population geneticsofH. gallinarumbased on mitochondrialcox2.
Keywords:Heterakis gallinarum;genetic variability;cox2;Sichuan
copyright:©2017 Informa UK Limited, trading as Taylor & Francis Group.
Mitochondrial DNA Part A, (2017):1-6. DOI:10.1080/24701394.2017.1334771
Read Full Text:http://dx.doi.org/10.1080/24701394.2017.1334771
