Absence of population genetic structure inHeterakis gallinarumof chicken from

Sichuan, inferred from mitochondrial cytochrome coxidase subunit I gene

Gu X, Zhu JY, Jian KL, Wang BJ, Peng XR, Yang GY, Wang T, Zhong ZJ, Peng KY

Abstract

Population genetics information provides a foundation for understanding the transmission and epidemiology of parasite and, therefore, may be used to assist in the control of parasitosis. However, limited available sequence information inHeterakis gallinarumhas greatly impeded the study in this area. In this study, we first investigated the genetic variability and genetic structure ofH. gallinarum. The 1325 bp fragments of the mitochondrial COX1 gene were amplified in 56 isolates ofH. gallinarumfrom seven different geographical regions in Sichuan province, China. The 56 sequences were classified into 22 haplotypes (H1-H22). The values of haplotype diversity (0.712) and nucleotide diversity (0.00158) in Sichuan population indicate a rapid expansion occurred from a relatively small, short-term effective population in the past. The haplotype network formed a distribution around H1 in a star-like topology, and the haplotypes did not cluster according to their geographical location. Similar conclusions could be made from MP phylogenetic tree. The Fst value (Fst<0 .16965) and amova analysis revealed that no significant genetic differentiation was observed among the seven different geographical populations. neutrality tests (tajima's d and fu's fs) and mismatch analysis indicated thatH. gallinarumexperienced a population expansion in the past. Our results indicated thatH. gallinarumexperienced a rapid population expansion in the past, and there was a low genetic diversity and an absence of population structure across the population.

Mitochondrial DNA. 2015 Sep 22:1-6. [Epub ahead of print]

Read full text: http://www.tandfonline.com/doi/abs/10.3109/19401736.2015.1079825?journalCode=imdn20#.VgU6jnhVeT8