Sequence Analysis of the Bs-Ag1 Gene ofBaylisascaris schroederifrom the Giant Panda and an Evaluation of the Efficacy of a RecombinantBaylisascaris schroederiBs-Ag1 Antigen in Mice
He G, Chen S, Wang T, Yan Y, Zhang Z, Li D, Yu H, Xie Y, Wang C, Gu X, Wang S, Peng X, Yang G.
Abstract
TheBaylisascarisschroederiinfection rate among wildgiantpandas may reach over 50% or even 100%, making it one of the leading causes of death from primary or secondary infection in wild populations. Until now, little was known about how protective immunity toB.schroederiinfection could be achieved. The present study was conducted to evaluate the immunogenicity and protectiveefficacyofrecombinantBs-Ag1fromB.schroederi, by cloning the full-lengthBs-Ag1geneofB.schroederiand expressing it in a heterologous host,Escherichia coliBL21. Inmicevaccinated with rBs-Ag1 coupled with Freund's complete adjuvant (FCA), there was a significant reduction (69.2%) in the recovery of challengedB.schroederiL3 compared with either nonvaccinated controls ormicevaccinated with FCA alone. Our study supports the use ofBs-Ag1as a potential candidate for vaccination againstB.schroederiinfection and provides basic data for further vaccination trials with mixtures of antigens (with Bs-Ag2 and Bs-Ag3) toB.schroederi.
Copyright © Mary Ann Liebert, Inc.
DNA Cell Biol.2012 Jul;31(7):1174-81. doi: 10.1089/dna.2011.1395. Epub 2012 Feb 17.
Read full text: http://online.liebertpub.com/doi/abs/10.1089/dna.2011.1395
