Cloning, expression and evaluation of the efficacy of a recombinantBaylisascaris schroederiBs-Ag3 antigen in mice
Wang T, He G, Yang G, Fei Y, Zhang Z, Wang C, Yang Z, Lan J, Luo L, Liu L.
Abstract
The geneBs-Ag3enconding aantigenof 37kDa fromBaylisascarisschroederi(giant panda isolates), as well as therecombinantBs-Ag3, obtained bycloningandexpressionof theBs-Ag3gene in heterologous hostEscherichia coliBL-21 (DE3), were used to evaluate their ability to induce immune protective responses in BALB/cmiceagainst L3-challenge infection in a mouse-B.schroederimodel.There was a significant reduction (62.91%) ofmicevaccinated with rBs-Ag3 coupled with Freund's complete adjuvant (FCA) in recovery of challengedB.schroederiL3 compared with either controls ormicevaccinated with FCA alone. Our data indicaterecombinantBs-Ag3may be a potential target as a vaccineantigenfor giant panda ascariasis.
Copyright © 2008 Published by Elsevier Ltd. All rights reserved.
Vaccine.2008 Dec 9;26(52):6919-24. doi: 10.1016/j.vaccine.2008.09.079. Epub 2008 Oct 16.
Read full text: http://www.sciencedirect.com/science/article/pii/S0264410X0801342X
