[18] Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

点击数:2023-10-17 09:30:18 

Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

Wei He, Ge Hao, Changming Xiong, Jie Xiao, Jiayan Pu, Hao Chen, Liwen Xu, Yuhua Zhu, Guangyou Yang

Abstract

Eimeria intestinalis is the most pathogenic species of rabbit coccidiosis, causing weight loss, diarrhea, and even acute death. The currently used anticoccidial drugs against E. intestinalis in rabbits are associated with drug resistance and residues. Immunological control might be a potential alternative. We cloned and expressed the E. intestinalis recombinant EF1α and EFG (rEi-EF1α and rEi-EFG, respectively). Rabbits were immunized subcutaneously every 14 days with 100 µg of rEi-EF1α and rEi-EFG and followed by 5 × 104 E. intestinalis sporulated oocysts orally challenge. Serum samples were collected every 7 days to measure the levels of specific antibodies and cytokines. On post-challenge day 14, rabbits were sacrificed and the anticoccidial index was evaluated. The rabbits of PBS challenged groups exhibited anorexia, diarrhea, marked intestinal wall thickening, and white nodules that formed patches, while rabbits from the rEi-EF1α or rEi-EFG challenged group exhibited milder symptoms. The rEi-EF1α group showed a 75.18% oocyst reduction and 89.01%wt gain; the rEi-EFG group had a 60.58% oocyst reduction and 56.04%wt gain. After vaccination, specific IgG levels increased and stayed high (P < 0.05). The IL-4 and IL-2 levels of rEi-EF1α immunized groups showed a significant increase after immunization (P < 0.05). Both rEi-EF1α and rEi-EFG could induce humoral and cellular immune responses. In contrast, rabbits immunized with rEi-EF1α were better protected from challenge by E. intestinalis than rEi-EFG.

Keywords: rabbit, Eimeria intestinalis, EF1α, EFG, recombinant protein, immune protection

Citation: He W, Hao G, Xiong C, Xiao J, Pu J, Chen H, Xu L, Zhu Y, Yang G. Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG. Infect Immun. 2023 Oct 12:e0020823. doi: 10.1128/iai.00208-23. Epub ahead of print. PMID: 37823630.

Read full text: https://journals.asm.org/doi/abs/10.1128/iai.00208-23?af=R